Abstract

Objective: The purpose of the study is to establish 5 Fluorouracil (5-FU) resistant OSCC Cell Lines to evaluate the characteristics of these cells towards the targeted drug. Methods: Fifty samples of oral squamous cells carcinoma have been taken from Hiroshima Univ ersity Dental Hospital. The non-treated patients were included in the study. Patients, who were formerly treated were excluded. The study has been conducted by the establishment of cell line and cell culture, 5-FU resistant OSCC (Oral Squamous Cell Carcino ma) cell lines, drug sensitivity assay, protein extraction and western blotting, quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction) analysis, and statistical analysis. Student’s t-test was applied to analyse the collected results. Results: Correlation between 5-FU and protein levels of TS (thymidylate synthase) and DPD (dihydropyrimidine dehydrogenase) in the observed cell lines have showed insignificant results. Cell lines of HSC-3R, HSC-4R, Ca-9-22R and Ho-1-N-1R showed resistance of 2.7, 2, 11.2 and 2.6 folds, respectively through PCR technique. Conclusion: OPRT (orotate phosphoribosyl transferase) and mRNA (Messenger RNA) have been reduced and resulted in low 5-FU. The study established 5 - Fluorouracil (5-FU) resistant oral squamous cell carcinoma (OSCC) cell lines to evaluate the characteristics of these cells towards the targeted drug.